A total of 24 ejaculates were obtained from 3 Mizo local boars (Zovawk), were used by split sample technique for evaluating the effect of four glycerol levels on quality of semen extended with Lactose egg yolk glycerol (LEYG) extender at equilibration and after freezing. The sperm motility at equilibration and after freezing was significantly higher (P<0.01) for 3 percent glycerol levels, than for 2 and 4 percent glycerol levels. The live sperm at equilibration and after freezing was significantly higher (P<0.01) for 3 percent glycerol levels than for 2 and 4 percent glycerol levels, and for 2 percent than for 3 percent glycerol level. The Hypo-Osmotic Sperm Swelling Test (HOSST) reacted spermatozoa at equilibration and after freezing was significantly (P<0.01) higher for 3 percent glycerol levels, than for 2 and 4 percent glycerol levels, and for 2 percent than for 3 percent glycerol level. The acrosomal integrity at equilibration and after freezing was significantly (P<0.01) higher for 3 percent glycerol levels, than for 2 and 4 percent glycerol levels, and for 2 percent than for 3 percent glycerol level. In conclusion, preservation of boar semen in LEYG extender using 3 percent glycerol found to be superior